Microsatellite marker based DNA fingerprinting of cotton (Gossypium spp.) hybrids and their parents

Cotton production in India by the vast majority comes from cotton hybrids whose genetic purity is of great significance seed chain and trade. Therefore, there a need to develop rapid, reliable reproducible technique assess as traditional, morphological traits-based ‘Grow-Out Test’ resource intensive, time consuming, tedious not an infallible procedure. In this regard, study was planned understand diversity among their parents also identify SSR markers for confirmation or hybridity. One intra-arboreum hybrid, CICR2 (DS 5 GMS × LD 327 Sel.), four intra-hirsutum viz., CSHH198 (CSH 19 CSH 8), CSHH238 (SH 2379 9Y PIL 8 CSHH243 2013 43), CSHH1862 (GMS 16A CB 33) one hirsutum barbadense Phule 388 (RHC-006 RHCb-001) along with respective parental lines were selected molecular characterization. Of total 215 surveyed, 60 conveyed polymorphism. The information polymorphic utilized divergence material. Maximum dissimilarity 0.66 noted between (Sel.), RHC-006 DS (GMS). Minimum 0.07 observed 33, followed 0.11 highly efficient capturing both intra-species inter-species level diversity. clustering factorial analysis  in congruence species Gossypium. diploid genotypes clustered separately distinctly rest genotypes. All found closely clustered. inter-specific its grouped closely. confirmed using identified [GH486, BNL1421, BNL3594, JESPR151 G. hybrids, CSHH198; GH486, BNL2449, JESPR151, TMB0436 CSHH238; JESPR152 GH527, BNL3812, TMB1484, TMB1645, NAU1190, BNL3816 hybrid 388]. purity. generated present about testing will greatly facilitate quality these thus, better production. Keywords: Cotton, DNA fingerprinting, Hybrids, Microsatellites, markers, Diversity